The level of animal mortality connected with transport for slaughter is reflective of the standard of welfare provided to the transported animals. Increased mortality during transport may, therefore, be a sign of violation of the obligations imposed on breeders and hauliers by the valid legislation regulating the commercial transport of animals. The aim of this study was to determine the mortality rate in culled dairy cows transported to slaughterhouses in the Czech Republic in the period from 2009 to 2014, and to investigate the possible relationship between transport- related mortality in dairy cows and the season of the year in which transport is conducted. The total mortality rate in culled dairy cows transported amounted to 0.207% in the monitored period. A statistically significant correlation was proven between mortality in culled dairy cows and season of the year. The highest mortality rate (0.284%) was recorded in the spring months, while the lowest mortality rate (0.137%) was found in the autumn.