Enterococcus spp. is the most controversial group of lactic acid bacteria that have been ascribed a beneficial or detrimental role in food and feed. The aim of our study was to monitor the occurrence of Enterococcus spp. in collected samples of meat and meat products. Enterococci originated from 34 meat and meat product samples collected from several producers in the Czech Republic, and a number of swab samples (n = 7) were also taken from the environment whit which meat comes into contact during manufacturing. Identification of isolates was performed by MALDI-TOF MS profiling. Totally 58 Enterococcus spp. included E. faecalis, E. faecium, E. gilvus, E. thailandicus, E. divriesei, E. hermanniensis, E. hirae, E. casseliflavus and E. durans were identified.