• Home
  • Archives
    • Archive 2020
      • Volume 01/2020
    • Archive 2019
      • Volume 01/2019
      • Volume 02/2019
    • Archive 2018
      • Volume 01/2018
    • Archive 2017
      • Volume 01/2017
    • Archive 2016
      • Volume 01/2016
      • Volume 02/2016
    • Archive 2015
      • Volume 01/2015
      • Volume 02/2015
    • Archive 2014
      • Volume 01/2014
      • Volume 02/2014
    • Archive 2013
      • Volume 01/2013
      • Volume 02/2013
    • Archive 2012
      • Volume 01/2012
      • Volume 02/2012
    • Archive 2011
      • Volume 01/2011
  • List of keywords
  • Submissions
    • Guidelines for Authors
    • Publication Ethics
  • Editorial Board
  • Contacts
MASO-INTERNATIONAL

Development of a method for analysis of tetracycline residues in cow’s milk by liquid chromatography/tandem mass spectrometry

Authors: František Buňka, Lucie Vydrová, Ludmila Zálešáková and Tomáš Šopík
Keywords: SPE, UPLC-MS/MS, food safety, tandem mass spectrometry, tetracycline, ultra performance liquid chromatography

An Ultra Performance Liquid Chromatography/tandem mass spectrometric (UPLC-MS/MS) multi-residue method for the simultaneous quantitative determination of tetracycline, chlortetracycline hydrochloride, oxytetracycline hydrochloride and doxycycline hydrochloride in milk has been developed in this work. Liquid-liquid extraction with trichloroacetic acid and McIlvaine buffer was used for the isolation of the selected residues from milk. The sample was centrifuged and solid-phase extraction with an OASIS® HLB extraction column was used to clean up the sample extract. The samples were analysed with the instrument operated in the Multiple Reaction Monitoring acquisition mode. UPLC separation was performed on a reversed-phase C18 column using gradient elution with a mobile phase consisting of acetonitrile and 20 mM formic acid. The tetracycline analytes were detected with a quadrupole mass spectrometer using positive ion electrospray ionisation. The confirmatory method has an acceptable detection limit and was validated according to the European requirements for veterinary drug residues according to Commission Decision 2002/657/ES. The recovery of tetracyclinesfrom milk ranged from 80.2 to 93.8% with relative standard deviation no larger than 7.3% for a concentration of 100 μg·l-1. Furthermore, this analytical method is a useful tool for food safety monitoring and controlling veterinary drug use.

Download full article
012016 03 10
Filename: 012016_03_10.pdf | Size: 1.3 MB | Downloads: 2081

Originaly published in MASO INTERNATIONAL Volume 01/2016

Search site

Current Issue

Current issue

MASO INTERNATIONAL 1/2020
ISSN 1805-5281 (printed)
ISSN 1805-529X (online)

Archive

Copyright © 2025 MASO-INTERNATIONAL.