A total of 1 554 945 head of cattle were transported for slaughter in the Czech Republic from 2009 to 2014, of which 1 935 (0.124%) died from causes connected with transport. Mortality rates in cattle differed statistically highly significantly (P < 0.01) according to the individual categories of cattle. The greatest mortality rate associated with transport was found in calves (0.297%), followed by dairy cows (0.219%) and heifers (0.062%). The lowest mortality rate was found in fattened cattle (0.018%) and steers (0.000%).