The aim of this study was to monitor the antimicrobial activity of honey. The effects of 44 samples of honey (29 blossom honeys, 15 honeydew honeys) were tested using an agar dilution method on 65 bacterial strains (genera Staphylococcus, Streptococcus, Listeria, Klebsiella, Escherichia and Paenibacillus). All the samples of honey inhibited the growth of all tested strains of L. monocytogenes, S. xylosus, P. larvae, P. alvei and Str. dysgalactiae and one strain of methicillin-sensitive S. aureus. 54.5% of the honeys showed high antimicrobial activity (inhibiting the growth of all the bacterial strains). This activity was higher in honeydew honeys (73.3%) than in blossom honeys (44.8%). Acacia and sunflower honey inhibited the growth of all the bacteria. The lowest antibacterial activity of the samples of honey was seen on Klebsiella spp.