The aim of this work was to assess methods for determining diastase activity in honey. Eight samples of honey from the retail network and 48 blends of honey with syrups (Apifood and Apivital) were tested using the Schade method and the Phadebas enzyme method. One of the eight samples of honey failed to meet the requirements of the Decree No. 76/2003 Coll. Diastase activity values of 11.13 units according to Schade, though 7.61 Schade units according to the Phadebas method, were measured in this sample. The two methods produced different diastase activity values, with the values according to Phadebas being on average 12.48% lower. A statistically significant difference (p < 0.05) was found between the methods. The cause of this difference in the results produced by the methods used was probably enzyme substrate specificity – the specific properties of the starch used as a substrate. The Schade method can be recommended for testing a small number of samples, while the Phadebas method is more suitable for serial analyses.