A large amount of by-product is created annually in the processing of watermelons and are being disposed of in most of the cases. The present study aimed to create a non-traditional product made of watermelon seed kernels and to analyse its stability and antioxidant potential during the 28 days of refrigerated storage. The obtained initial value for TPC was 0.94 mg GAE/g, for FRAP 3.79 μmol/g Trolox and DPPH inhibition was 28 %. The MDA was found at the level of 2.08 μg/g. After 28 days of storage, TPC value dropped to 0.57 mg GAE/g, FRAP to 2.52 μmol/g Trolox, while no statistically significant change was observed for parameters of DPPH and MDA. It can be concluded that even though the obtained product was contained in a large extent with oil rich in unsaturated fatty acids that are susceptible to oxidation, no increase in MDA as an indicator of lipid oxidation was found. The created spread represents a product with great potential for development for human consumption.